QPCR: Difference between revisions
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medium | |||
The medium will be prepared in 60 l batches. The medium is prepared according to Ian Probert's K/2(-Tris-Si) recipe. Seawater is collected at point B in the bay of Villefranche, filtered on a 10 µm filter and stored in 60 l tanks. Solutions are prepared in Antoine Sciandra's lab and stored in the fridge there. The 20 l Nalgene tanks are rinsed with tap water, distilled water and finally MilliQ water. The seawater is filtered on a 0.2 µm filter and transfered to the Nalgene tanks. Then the different solutions, except the vitamin solution, are added to the filtered seawater. | |||
This is the recipe for 40 l. The alternative solutions mentioned are solutions which are already present in Antoine Sciandra's lab. | |||
solutions | |||
to 39.780 l filtered seawater add | |||
20 ml NaNO3 solution (48.9642 g per l H2O) | |||
20 ml KH2PO4 solution (4.8992 g per l H2O) | |||
20 ml FeEDTA solution | |||
20 ml trace metal solution | |||
40 ml f/2 vitamin solution | |||
Revision as of 02:33, 31 May 2006
medium The medium will be prepared in 60 l batches. The medium is prepared according to Ian Probert's K/2(-Tris-Si) recipe. Seawater is collected at point B in the bay of Villefranche, filtered on a 10 µm filter and stored in 60 l tanks. Solutions are prepared in Antoine Sciandra's lab and stored in the fridge there. The 20 l Nalgene tanks are rinsed with tap water, distilled water and finally MilliQ water. The seawater is filtered on a 0.2 µm filter and transfered to the Nalgene tanks. Then the different solutions, except the vitamin solution, are added to the filtered seawater. This is the recipe for 40 l. The alternative solutions mentioned are solutions which are already present in Antoine Sciandra's lab. solutions to 39.780 l filtered seawater add
20 ml NaNO3 solution (48.9642 g per l H2O) 20 ml KH2PO4 solution (4.8992 g per l H2O) 20 ml FeEDTA solution 20 ml trace metal solution 40 ml f/2 vitamin solution
