QRT-PCR
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See Q-PCR for much useful information.
This page focuses on information specific to quantitative reverse transcriptase PCR rather than quantitative PCR in general.
Notes
- The most commonly used specialist reverse transcriptase enzyme for cDNA production is AMV reverse transcriptase. It has RNase H activity (so that RNA molecules are only transcribed once) and has a high temperature stability (to reduce RNA secondary structure and nonspecific primer annealing).
