QRT-PCR/Single tube: Difference between revisions
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(Real-time RT-PCR moved to Quantitative reverse transcriptase PCR: naming here is difficult but I think RT RT PCR is confusing; real-time doesn't tell you much; Q tells you that this is all about quantification;) |
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This protocol describes one-step real time quantitivie PCR to quantify relative levels of RNA in | |||
== Starting Materials == | |||
* Validated PCR primers that are efficient over the range of RNA that you are assaying. | |||
* | |||
== Basic Principle == | |||
* RNA is reverse transcribed in a single well. | |||
* PCR reaction procedes. The levels of template are quantified during each cycle. | |||
== Protocol == | |||
== Analysis == | |||
Delta-Delta Ct Method. See Livak KJ, Schmittgen. Methods '''25''' 402-408 (2001) | |||
Revision as of 13:10, 26 March 2007
This protocol describes one-step real time quantitivie PCR to quantify relative levels of RNA in
Starting Materials
- Validated PCR primers that are efficient over the range of RNA that you are assaying.
Basic Principle
- RNA is reverse transcribed in a single well.
- PCR reaction procedes. The levels of template are quantified during each cycle.
Protocol
Analysis
Delta-Delta Ct Method. See Livak KJ, Schmittgen. Methods 25 402-408 (2001)
