QRT-PCR/Two tubes

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(New page: The first tube produces cDNA from an RNA sample. The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of...)
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The first tube produces cDNA from an RNA sample.  The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules.
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==Overview==
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The first tube produces cDNA from an RNA sample.  The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules. The reaction is stopped by heating and an aliquot is taken to serve as the template for a sequence specific [[qPCR]] step.
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The second tube is sequence specific [[qPCR]] step.
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==References==
==References==

Revision as of 15:15, 9 October 2007

Overview

The first tube produces cDNA from an RNA sample. The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules. The reaction is stopped by heating and an aliquot is taken to serve as the template for a sequence specific qPCR step.

References

  1. Matthew B. Avison. Measuring gene expression. New York, NY: Taylor & Francis Group, 2007. isbn:0415374723. [MeasuringGeneExpression]
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