Quantification of glucose: Difference between revisions
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==Introduction== | ==Introduction== | ||
This method is used to quantify glucose using a YSI 2700 Select biochemistry analyzer. The maximum concentration that should be read is 3 grams per liter, so dilutions may need to be made before analysis. | |||
==Method== | ==Method== | ||
SET-UP | ===SET-UP=== | ||
# Rinse out Buffer container and fill to line with YSI 2357 Buffer solution | # Rinse out Buffer container and fill to line with YSI 2357 Buffer solution | ||
# Hit [Menu} then {2-MeasParameter] then [2-CalMethod] then [1-One Station] | # Hit [Menu} then {2-MeasParameter] then [2-CalMethod] then [1-One Station] | ||
Line 12: | Line 14: | ||
RUN SAMPLES | ===RUN SAMPLES=== | ||
# Hit [Run] and wait for system to complete calibration | # Hit [Run] and wait for system to complete calibration | ||
# When Ready screen appears, hit [Sample] | # When Ready screen appears, hit [Sample] | ||
# Present sample to needle in Station S3 without touching needle to tube | # Present sample to needle in Station S3 without touching needle to tube | ||
# Hit [Sample] and wait for needle to retract | # Hit [Sample] and wait for needle to retract | ||
# Results will print out at the completion of run | |||
# To continue testing samples, hit [Sample] when Ready screen appears | |||
*Note: After 5-7 runs, system will automatically calibrate. | *Note: After 5-7 runs, system will automatically calibrate. | ||
==Notes== | ==Notes== | ||
*Glucose standard good for 30 days after opening | |||
* replace tubing every 6 months | |||
* Glucose membrane needs to be replaced every 21 days (Ethanol membrane is every 5 days) | |||
==References== | ==References== |
Latest revision as of 14:02, 4 November 2011
Introduction
This method is used to quantify glucose using a YSI 2700 Select biochemistry analyzer. The maximum concentration that should be read is 3 grams per liter, so dilutions may need to be made before analysis.
Method
SET-UP
- Rinse out Buffer container and fill to line with YSI 2357 Buffer solution
- Hit [Menu} then {2-MeasParameter] then [2-CalMethod] then [1-One Station]
- Hit [Enter] twice to return to Measurement Parameter Set up Screen
- Hit [3-Black]
- Make sure Current BLACK probe chem is set to NONE. If it isn't, hit [1-Next chemistry] until NONE appears and hit [ENTER] to save
- Hit [4-White] and set Current WHITE probe chem to Dextrose
- Hit [Enter] 4 times to return to Measurement Parameter Set up Screen
- Hit [Menu] to return to home screen
RUN SAMPLES
- Hit [Run] and wait for system to complete calibration
- When Ready screen appears, hit [Sample]
- Present sample to needle in Station S3 without touching needle to tube
- Hit [Sample] and wait for needle to retract
- Results will print out at the completion of run
- To continue testing samples, hit [Sample] when Ready screen appears
- Note: After 5-7 runs, system will automatically calibrate.
Notes
- Glucose standard good for 30 days after opening
- replace tubing every 6 months
- Glucose membrane needs to be replaced every 21 days (Ethanol membrane is every 5 days)