Quantification of nucleic acids
From OpenWetWare
Nucleic acids are typically quantitated based on their absorbance of ultraviolet light. Each base has a different absorbance maximum, with the average being at 260 nm. Ambion has a good site that gives a variety of parameters for nucleotides and nucleic acids [1].
Quantification of RNA
- Northern blotting (or RNA blotting)
- ribonuclease protection assays (RPA)
- Q-PCR or more specifically QRT-PCR
Quantification of DNA
- Southern blotting (or DNA blotting)
- Q-PCR
