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tools dna/protein analysis: sequence converter dna clean-up protein clean-up reverse complement translate dna predict protein mw protein motif scan restriction analysis oligos: oligo analysis primer3 duplex analysis pcr mastermix calculator blasts/alignments: pairwise dna alignment protein alignment blast alignment clustalw - multiple alignments blastn blastp arabidopsis tools: tair nasc plants-ubq salk t-dnas genevestigator efp-browser/expression angler/etc. arex mapman web mirna designer natural variation/polymorphisms: msqt - multiple snp query tool masc snp database caps finder polymorph - reseq. snps atprimer ecotypes calculators: vector:insert ratios molar conversions - moles ↔ weight concentration conversions - molar units ↔ weight/volume

protocols arabidopsis dna isolation - quick and dirty arabidopsis transformation ems mutagenesis colony pcr cacl2-competent cells e.coli electrocompetent cells e.coli agrobacterium plasmid miniprep ats plates iaa extraction and quantification ligation northern western e.coli transformation chemical