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resources

• primer lists
  
• enzyme lists
  
• seeds
  
• ordering
  

papers/lab meetings/seminars papers of the month lab meeting/journal club ipb progress reports/seminars SFB648 seminars

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online tools dna/protein analysis: sequence converter dna clean-up protein clean-up reverse complement translate dna predict protein mw protein motif scan restriction analysis genetic code amino acid structures plasmapper oligos: oligo analysis primer3plus duplex analysis blasts/alignments: pairwise dna alignment protein alignment blast alignment clustalw - multiple alignments blastn blastp Vicogenta - comparing genomes to arabidopsis arabidopsis tools: arabidopsis database collection tair nasc 101 ways to grow arabidopsis plants-ubq salk t-dnas genevestigator efp-browser/expression angler/etc. GEB - gene expression browser for arabidopsis tiling array expression atlas arex mapman web mirna designer tile viz tiling array express 1001 genomes POLYMORPH reseq SNPs arabidopsis hormone database JIC microsatellite mapping markers Vicogenta - comparing genomes to arabidopsis AmiGO - Gene Ontology PLAZA medicago tools: medicago gene expression atlas medicago database natural variation/polymorphisms: msqt - multiple snp query tool masc snp database caps finder polymorph - reseq. snps atprimer ecotypes JIC microsatellite mapping markers Marker Tracker Bergelson A.thaliana Resources calculators: vector:insert ratios molar conversions - moles ↔ weight concentration conversions - molar units ↔ weight/volume pcr mastermix calculator buffer calculator pcr mastermix calculator Konzrechner

white board AG Quint - Lab Blog DFG Science TV: Forscher stellen ihre eigenen Projekte vor Naturally Obsessed: The making of a Scientist - documentary historical papers of the royal society professor science free PC software for molecular biologists Six Important Factors for Reverse Transcription Video: Jede Zelle ... Video: GFP-tagged HIV infiziert T-Zelle The Large Hadron Rap medicago gene expression atlas the postdoc interview craig venter's grand vision on synthetic genomics → video Kits and their unique role in molecular biology: a brief retrospective recycling electroporation cuvettes pimp your plasmid growth medium buffer calculator Control Your Lab Computers From Home run faster gels → new electrophoresis buffers how-to-become-a-world-expert-in-your-field → artikel quick and dirty screening for cloned inserts awesome video → cellular visions: the inner life of a cell

lab protocols → check also openwetware protocols e. coli cacl2-competent cells e.coli e.coli transformation chemical electrocompetent cells e.coli + electroporation colony pcr agros electrocompetent A.rhizogenes cells + electroporation agrobacterium plasmid miniprep arabidopsis southern blot arabidopsis dna isolation - quick and dirty arabidopsis transformation arabidopsis protoplast transformation arabidopsis rna isolation protocols (including siliques) transient transformation and expression kanamycin selection of transgenic seeds selection of transgenic seeds on basta plates ems mutagenesis ats plates iaa extraction and quantification yeast → for general yeast handling follow Clontech Yeast Protocols Handbook → for Y2H follow Clontech Matchmaker Library Construction & Screening Kit genomic dna non-lethal ß-galactosidase assay Short version ß-galactosidase assay plasmid prep yeast transformation yeast western blot mating in 24-well plates yeast media general ligation (sticky ends) northern western