Quint Lab:arabidopsis protoplast transformation: Difference between revisions
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==arabidopsis protoplast transformation== | ==arabidopsis protoplast transformation== | ||
''Advice'' | ''Advice'' |
Latest revision as of 06:40, 16 October 2008
arabidopsis protoplast transformationAdvice
→ adjust pH with KOH
→ adjust pH 9,0 (Tris or Bicine for buffering)
→ autoclave
→ adjust pH 5,5 with NaOH
→ shaking incubation for 2-4 h at RT or o.N. at 4°C |