Quint Lab:enzyme lists

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(→Restriktionsenzyme)
(→Restriktionsenzyme)
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| PaeI || SphI || 1x Buffer B || 37°C || 65°C 20' || || || ||
| PaeI || SphI || 1x Buffer B || 37°C || 65°C 20' || || || ||
|-  
|-  
-
| PciiI || || NEB3 || 37°C || 80°C 20' || ''' yes''' || || ||
+
| PciI || PscI || NEB3 || 37°C || 80°C 20' || ''' yes''' || || ||
|-  
|-  
| PsyI || Tth111I || 1x Buffer B || 37°C || 80°C 20' || || || ||
| PsyI || Tth111I || 1x Buffer B || 37°C || 80°C 20' || || || ||
Line 138: Line 138:
|-  
|-  
| SmaI || || 1x Tango || '''30°C''' || 65°C 20' || || || ||
| SmaI || || 1x Tango || '''30°C''' || 65°C 20' || || || ||
 +
|-
 +
| ScrFI || Bme1390I || NEB4 || 37°C || 65°C 20' || || || ||
|-  
|-  
| SpeI Fast Digest (?)|| BcuI || 1x Fast Digest || 37°C || no || || || ||
| SpeI Fast Digest (?)|| BcuI || 1x Fast Digest || 37°C || no || || || ||

Revision as of 06:16, 27 March 2012

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Restriktionsenzyme

enzyme isoschizomeres buffer temperature heat inactivated add BSA add SAM add Oligonucleotide star activity


AatII (?) 1x NEBuffer 4 37°C 65°C 20'
AatII 1x Tango 37°C 65°C 20'
Alw26I BsmAI 1x Tango 37°C 65°C 20'
Alw26I Fast Digest (?) BsmAI 1x Fast Digest 37°C 65°C 20'
AseI VspI NEB3 37°C 65°C 20'
BamHI Buffer BamHI 37°C 80°C 20'
BamHI Fast Digest 1x Fast Digest 37°C 80°C 5'
BauI BssSI 1 x Tango 37°C 65°C 20'
BbvI BseXI 1x NEBuffer 2 37°C 65°C 20'
BbsI Fast Digest BpiI 1x Fast Digest 37°C 65°C 10'
BcuI SpeI 1x Tango 37°C no
BfuI BciVI 1x Buffer BfuI 37°C 80°C 20'
BpiI BbsI 1x Buffer G 37°C 65°C 20'
BglII Fast Digest 1x Fast Digest 37°C no
BseGI FokI 1x Tango 55°C 80°C 20'
BseMII BspCNI 1x Tango 55°C 80°C 20' yes
BseNI BsrI 1x Buffer B 65°C 80°C 20'
BseRI 1x NEBuffer 4 37°C 65°C 20'
Bsp119I BstBI 1x Tango 37°C 80°C 20'
Bsp1407I Fast Digest BsrGI 1x Fast Digest 37°C 80°C 10'
BspOI BmtI 1x Buffer O 37°C 80°C 20'
BveI BspMI 1x Buffer O 37°C 65°C 20' yes *
Cfr10I BsrFI Buffer Cfr10I 37°C no *
DpnI Fast Digest BsrFI 1x Fast Digest 37°C 80° 5' *
EagI-HF Eco52I 1x NEBuffer 4 37°C 65°C 20' *
Eam1104I EarI 1x Tango 37°C 65°C 20'
Eco31I Fast Digest 1x Fast Digest 37°C 65°C 5'
Eco32I EcoRV 1x Buffer R 37°C 80°C 20'
Eco47I AvaII 1x Buffer R 37°C 65°C 20'
Eco147I StuI 1x Buffer B 37°C 80°C 20'
EcoRI Buffer EcoRI 37°C 65°C 20'
EcoRI Fast Digest 1x Fast Digest 37°C 80°C 5'
EcoRV Eco32I NEB3 37°C 80°C 20' yes
EcoRV Fast Digest 1x Fast Digest 37°C no
FspI NsbI 1x NEBuffer 4 37°C 65°C 20'
HindIII NEB2 37°C 65°C 20'
Hin6I HinP1I 1x Tango 37°C no
Hpy188I NEB4 37°C 65°C 20'
HpyF3I DdeI 1x Tango 37°C 65°C 20'
HpyF10VI MwoI 1x Tango 37°C 80°C 20'
KpnI ReACT4 37°C
KpnI Fast Digest 1x Fast Digest 37°C 80°C 5'
Kpn2I BspEI 1 x Tango 55°C 80°C 20'
KspAI HpaI 1x Buffer B 37°C 65°C 20'
MboI 1x Buffer R 37°C 65°C 20'
MboII 1x Buffer B 37°C 65°C 20'
MnlI 1x Buffer G 37°C 65°C 20'
MvaI BstNI 1x Buffer R 37°C no
NcoI 1x Tango 37°C 65°C 20'
NcoI Fast Digest 1x Fast Digest 37°C 65°C 15'
PacI (?) NEB1 37°C 65°C 20' yes
PaeI SphI 1x Buffer B 37°C 65°C 20'
PciI PscI NEB3 37°C 80°C 20' yes
PsyI Tth111I 1x Buffer B 37°C 80°C 20'
PvuII NEB2 37°C no
SacI Buffer SacI 37°C 65°C 20'
SacII Cfr42I NEB4 37°C 65°C 20'
SalI SuRE/Cut Buffer H 37°C *
SapI 1x NEBuffer 4 37°C 65°C 20'
SmaI 1x Tango 30°C 65°C 20'
ScrFI Bme1390I NEB4 37°C 65°C 20'
SpeI Fast Digest (?) BcuI 1x Fast Digest 37°C no
TaqI Buffer TaqI 65°C no
VspI AseI 1x Buffer O 37°C 65°C 20'
XhoI 1x Buffer R 37°C 80°C 20'
XhoI Fast Digest 1x Fast Digest 37°C 80°C 5'
(?) = Achtung sehr wenig

DNA-modifying

enzym in stock? supplier, bestnr. jahr
T4 DNA ligase y z
RevertAid M-MuLV Reverse Transcriptase ja Fermentas, EP0442 2008
x y z
x y z
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