RNA extraction

This is a general protocol page for extracting RNA from samples.

Specific Protocols

• Endy:RNA extraction
• Extraction of RNA from tissues in English
• Extraction of RNA from tissues in French
• Gattuso:RNA extraction

Notes

Some general notes on isolating RNA.

These notes are focused on bacterial cells but please contribute to include information about plant, mammalian and other cells etc.

1. Centrifugation of cells can induce a stress response in cells altering transcript levels (potentially unequally across different transcripts).
2. "RNAprotect" is a reagent used to stabilize RNA content in bacterial cells grown in liquid culture.
   • Works best if cells are grown in minimal media. Works worst in LB liquid culture due to complex components.

Related information

See RNA.

Reference

1. ISBN:0415374723