RNA extraction - Revision history

Colin Archer: /* Commercially available kits */

2012-08-22T05:50:07Z

Commercially available kits

←Older revision		Revision as of 05:50, 22 August 2012
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	===Commercially available kits===		===Commercially available kits===
-	* Qiagen ~~RNA easy~~ kits (silica column tubes) are the most widely recommended. It is more expensive than other kits but is good for transcriptomics.	+	* Qiagen RNeasy kits (silica column tubes) are the most widely recommended. It is more expensive than other kits but is good for transcriptomics.
-	* The column binding system of RNAeasy is faster and without harmful chemical but it has a lower maximum yield (due to the binding capacity of the columns).	+	* The column binding system of RNAeasy is faster and without harmful chemical but it has a lower maximum yield (due to the binding capacity of the columns) and will remove RNA smaller than ~200 nt.
	* All the kits are suitable for applications like RT-PCR as long as you check for and remove contaminants from RNA preparation.		* All the kits are suitable for applications like RT-PCR as long as you check for and remove contaminants from RNA preparation.

2011-03-12T16:22:17Z

←Older revision		Revision as of 16:22, 12 March 2011
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	===Silica matrices===		===Silica matrices===
-	*Very small species (< 200nt) do not bind to silica matrices.	+	*Very small species (< 200nt) do not bind to silica matrices (except for Norgen columns which get miRNAs).

	===Commercially available kits===		===Commercially available kits===

2009-06-17T16:57:31Z

TRIzol or tri followed by chloroform and precipitation

←Older revision		Revision as of 16:57, 17 June 2009
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	* phenol and chloroform are potentially harmful reagents (handle under the hood)		* phenol and chloroform are potentially harmful reagents (handle under the hood)

-	see ~~also~~:	+	For detailed protocols see:
	* [[RNA extraction using trizol/tri]]		* [[RNA extraction using trizol/tri]]
	* [[Trizol extraction for RNA and DNA]]		* [[Trizol extraction for RNA and DNA]]

2009-06-17T16:57:06Z

TRIzol or tri followed by chloroform and precipitation: links

←Older revision		Revision as of 16:57, 17 June 2009
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	* RNA is protected by the reagent during the extraction procedure		* RNA is protected by the reagent during the extraction procedure
	* phenol and chloroform are potentially harmful reagents (handle under the hood)		* phenol and chloroform are potentially harmful reagents (handle under the hood)
		+
		+	see also:
		+	* [[RNA extraction using trizol/tri]]
		+	* [[Trizol extraction for RNA and DNA]]

	===Anion-exchange matrices===		===Anion-exchange matrices===

2009-01-16T10:07:19Z

removed frame around image

←Older revision		Revision as of 10:07, 16 January 2009
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-	[[Image:Hub icon.png\|right\|~~thumbnail\|'''~~protocol hub~~'''~~]]	+	[[Image:Hub icon.png\|right\|protocol hub]]

	This is a general protocol page for extracting RNA from cells. The page also links to individual RNA extraction protocols (see at the end). Some sections link to separate pages with more in-depth explanations (see main page..).		This is a general protocol page for extracting RNA from cells. The page also links to individual RNA extraction protocols (see at the end). Some sections link to separate pages with more in-depth explanations (see main page..).

2008-06-26T11:02:49Z

←Older revision		Revision as of 11:02, 26 June 2008
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-	[[Image:Hub icon.png\|right\|thumbnail\|protocol hub]]	+	[[Image:Hub icon.png\|right\|thumbnail\|'''protocol hub''']]

	This is a general protocol page for extracting RNA from cells. The page also links to individual RNA extraction protocols (see at the end). Some sections link to separate pages with more in-depth explanations (see main page..).		This is a general protocol page for extracting RNA from cells. The page also links to individual RNA extraction protocols (see at the end). Some sections link to separate pages with more in-depth explanations (see main page..).

2008-06-26T11:00:44Z

illustration

←Older revision		Revision as of 11:00, 26 June 2008
Line 1:		Line 1:
		+	[[Image:Hub icon.png\|right\|thumbnail\|protocol hub]]
		+
	This is a general protocol page for extracting RNA from cells. The page also links to individual RNA extraction protocols (see at the end). Some sections link to separate pages with more in-depth explanations (see main page..).		This is a general protocol page for extracting RNA from cells. The page also links to individual RNA extraction protocols (see at the end). Some sections link to separate pages with more in-depth explanations (see main page..).

2008-02-14T17:53:35Z

Commercially available kits

←Older revision		Revision as of 17:53, 14 February 2008
Line 29:		Line 29:

	===Commercially available kits===		===Commercially available kits===
-	*Qiagen RNA easy kits (silica column tubes) are the most widely recommended. It is more expensive than other kits but is good for transcriptomics. All the kits are suitable for applications like RT-PCR as long as you check for and remove contaminants from RNA preparation.	+	* Qiagen RNA easy kits (silica column tubes) are the most widely recommended. It is more expensive than other kits but is good for transcriptomics.
		+	* The column binding system of RNAeasy is faster and without harmful chemical but it has a lower maximum yield (due to the binding capacity of the columns).
		+	* All the kits are suitable for applications like RT-PCR as long as you check for and remove contaminants from RNA preparation.

	==Harvesting cells==		==Harvesting cells==

2008-02-14T17:41:31Z

Quantification

←Older revision		Revision as of 17:41, 14 February 2008
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	* Absorption measurement depends on the buffer (use the same buffer as blank)		* Absorption measurement depends on the buffer (use the same buffer as blank)

-	{\| ~~class="wikitable"~~	+	{\| {{table}}
	\|-		\|-
-	!diluent	+	! style="background:lightgrey"\|diluent
-	!A260/A280	+	! style="background:lightgrey"\|A260/A280
	\|-		\|-
	\|DEPC water (pH 5-6)		\|DEPC water (pH 5-6)
	\|1.60		\|1.60
	\|-		\|-
-	\|Nuclease-free water (pH 6-7)	+	\|Nuclease-free water (pH 6-7)
	\|1.85		\|1.85
	\|-		\|-
	\|TE (pH 8.0)		\|TE (pH 8.0)
-	\|2.14	+	\|2.14
		+	\|-
	\|}		\|}

2008-02-14T17:35:27Z

grouped further reading and specific protocols under appendix to make the TOC easier to read

←Older revision		Revision as of 17:35, 14 February 2008
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	*Isopropanol precipitation coupled with a 70% v/v ethanol wash of the pellet can remove salts.		*Isopropanol precipitation coupled with a 70% v/v ethanol wash of the pellet can remove salts.

-	==See also==	+	== Appendix ==
		+
		+	=== See also ===
	* [[RNA]]		* [[RNA]]

-	=~~=Reference==~~	+	===Specific Protocols===
-	~~<biblio>~~	+
-	~~#MeasuringGeneExpression isbn=0415374723~~	+
-	~~</biblio>~~	+
-		+
-	==Specific Protocols==	+
	*[[Endy:RNA extraction]]		*[[Endy:RNA extraction]]
	*[[Etchevers:RNA tissues\|Extraction of RNA from tissues in English]]		*[[Etchevers:RNA tissues\|Extraction of RNA from tissues in English]]
	*[[Etchevers:Extraction ARN francais\|Extraction of RNA from tissues in French]]		*[[Etchevers:Extraction ARN francais\|Extraction of RNA from tissues in French]]
	*[[Gattuso:RNA extraction]]		*[[Gattuso:RNA extraction]]
		+
		+	===Reference===
		+	<biblio>
		+	#MeasuringGeneExpression isbn=0415374723
		+	</biblio>
		+
		+	=== External links ===
		+	* [http://www.ambion.com/techlib/tn/94/949.html Ambion tips on RNA quantification]