RNA extraction using trizol/tri
From OpenWetWare
RNA extraction with TRIZOL (Invitrogen product name) or the equivalent TRI (Sigma-Aldrich product name) is a common method of total RNA extraction from cells. It takes slightly longer than column-based methods like RNAeasy but it has higher capacity and can yield more RNA.
Principle
Reagents
Steps
Common mistakes
See also
- RNA extraction (central, general page)
External links
Reagents
- TRIZOL reagent (Invitrogen), TRIZOL on wikipedia
- TRI reagent (Sigmal Aldrich)
Protocols
- RNA extraction with TRIZOL (Stanford)
- RNA extraction with TRIZOL (Uni Florida)
- Troubleshooting guide for TRIZOL extraction (Uni Toronto)
Tips