Random Lab Methods: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 3: | Line 3: | ||
==Rapid Isolation of RNA from Conifer Needles== | ==Rapid Isolation of RNA from Conifer Needles== | ||
[[Conifer_RNA_prep]]. Conifers join a long list of 'recalcitrant' plants that are difficult for RNA extraction, and fail using "traditional" RNA extraction kits. We use a modification of the method by LeProvost, 2007. | [[Conifer_RNA_prep]]. Conifers join a long list of 'recalcitrant' plants that are difficult for RNA extraction, and fail using "traditional" RNA extraction kits. We use a modification of the method by LeProvost, 2007. | ||
==Isolation of Polyadenylated mRNA using Sera-Mag Oligo(dT) beads== | ==Isolation of Polyadenylated mRNA using Sera-Mag Oligo(dT) beads== |
Revision as of 00:19, 1 December 2010
Rapid Isolation of RNA from Conifer Needles
Conifer_RNA_prep. Conifers join a long list of 'recalcitrant' plants that are difficult for RNA extraction, and fail using "traditional" RNA extraction kits. We use a modification of the method by LeProvost, 2007.
Isolation of Polyadenylated mRNA using Sera-Mag Oligo(dT) beads
mRNA_Prep. There are many methods for isolating mRNA from total RNA. We have had excellent results with Sera-Mag Oligo(dT) beads. We use this approach for constructing Illumina mRNA-Seq libraries, but it should be useful for any application that demands rRNA-depleted mRNA.
Rapid Isolation of DNA from Conifer Needles
DNA_Seq Prep. Conifers join a long list of 'recalcitrant' plants that are difficult for DNA extraction. We use a modification of the Fast-Prep method.