Random Lab Methods: Difference between revisions

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==Isolation of polyA mRNA with Sera-Mag Oligo(dT) beads==
==Isolation of poly(A) mRNA with Sera-Mag Oligo(dT) beads==
[[mRNA_Prep]]. There are many methods for isolating mRNA from total RNA. We have had excellent results with Sera-Mag Oligo(dT) beads. We use this approach for constructing Illumina mRNA-Seq libraries, but it should be useful for any application that demands rRNA-depleted mRNA.
[[mRNA_Prep]]. There are many methods for isolating mRNA from total RNA. We have had excellent results with Sera-Mag Oligo(dT) beads. We use this approach for constructing Illumina mRNA-Seq libraries, but it should be useful for any application that demands rRNA-depleted mRNA.


==Rapid Isolation of DNA from Conifer Needles==
==Rapid Isolation of DNA from Conifer Needles==
[[DNA_Seq Prep]]. Conifers join a long list of 'recalcitrant' plants that are difficult for DNA extraction. We use a modification of the Fast-Prep method.
[[DNA_Seq Prep]]. Conifers join a long list of 'recalcitrant' plants that are difficult for DNA extraction. We use a modification of the Fast-Prep method.

Revision as of 00:20, 1 December 2010

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Rapid Isolation of RNA from Conifer Needles

Conifer_RNA_prep. Conifers join a long list of 'recalcitrant' plants that are difficult for RNA extraction, and fail using "traditional" RNA extraction kits. We use a modification of the method by LeProvost, 2007.


Isolation of poly(A) mRNA with Sera-Mag Oligo(dT) beads

mRNA_Prep. There are many methods for isolating mRNA from total RNA. We have had excellent results with Sera-Mag Oligo(dT) beads. We use this approach for constructing Illumina mRNA-Seq libraries, but it should be useful for any application that demands rRNA-depleted mRNA.

Rapid Isolation of DNA from Conifer Needles

DNA_Seq Prep. Conifers join a long list of 'recalcitrant' plants that are difficult for DNA extraction. We use a modification of the Fast-Prep method.