Random Lab Methods: Difference between revisions
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==Rapid Isolation of RNA from Conifer Needles== | ==Rapid Isolation of RNA from Conifer Needles== | ||
[[Conifer_RNA_prep]]. Conifers join a long list of 'recalcitrant' plants that are difficult for RNA extraction, and fail using "traditional" RNA extraction kits. We use a modification of the method by | [[Conifer_RNA_prep]]. Conifers join a long list of 'recalcitrant' plants that are difficult for RNA extraction, and fail using "traditional" RNA extraction kits. We use a modification of the method by Tai et al, 2004. | ||
==Isolation of poly(A) mRNA with Sera-Mag Oligo(dT) beads== | ==Isolation of poly(A) mRNA with Sera-Mag Oligo(dT) beads== | ||
Revision as of 17:56, 6 December 2010
Rapid Isolation of RNA from Conifer Needles
Conifer_RNA_prep. Conifers join a long list of 'recalcitrant' plants that are difficult for RNA extraction, and fail using "traditional" RNA extraction kits. We use a modification of the method by Tai et al, 2004.
Isolation of poly(A) mRNA with Sera-Mag Oligo(dT) beads
mRNA_Prep. There are many methods for isolating mRNA from total RNA. We have had excellent results with Sera-Mag Oligo(dT) beads. We use this approach for constructing Illumina mRNA-Seq libraries, but it should be useful for any application that demands rRNA-depleted mRNA.
Rapid Isolation of DNA from Conifer Needles
DNA_Seq Prep. Conifers join a long list of 'recalcitrant' plants that are difficult for DNA extraction. We use a modification of the Fast-Prep method.
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