Rao:LB: Difference between revisions

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Line 5: Line 5:
* Yeast Extract: 5g
* Yeast Extract: 5g
* Tryptone: 10g
* Tryptone: 10g
* NaCl: 10g
* NaCl: 10g (5g if you're in S. typhimurium)


pH to 7, Store at 4-30 C
pH to 7, Store at 4-30 C


[[Rao:Protocols|Back to Protocols]]
[[Rao:Protocols|Back to Protocols]]

Latest revision as of 15:43, 2 December 2007

Description

LB (or Luria broth or Luria-Bertani media) is a standard rich media suitable for growth of many bacteria species.

Recipe

Per 1L of H2O:

  • Yeast Extract: 5g
  • Tryptone: 10g
  • NaCl: 10g (5g if you're in S. typhimurium)

pH to 7, Store at 4-30 C

Back to Protocols