Rao:Lab Tasks: Difference between revisions
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=Autoclaving= | =Autoclaving= | ||
*All dry items (tips, flasks, tubes, etc.) - 20 minutes steam, 20 minutes drying | |||
*All liquids (plate agar, media, solutions) - 20 minutes steam | |||
*All waste (old plates, etc.) - Must be in bio-hazard bag. 1 hr in big autoclave. | |||
=Dish washing= | =Dish washing= | ||
*Wash with dissolved powdered detergent. Rinse with DI water (Milli-Q if you're worried). | |||
*Do not wash out water bottles with detergent! | |||
*Do not wash empty 10% glycerol bottles with detergent! |
Latest revision as of 10:19, 30 November 2006
Autoclaving
- All dry items (tips, flasks, tubes, etc.) - 20 minutes steam, 20 minutes drying
- All liquids (plate agar, media, solutions) - 20 minutes steam
- All waste (old plates, etc.) - Must be in bio-hazard bag. 1 hr in big autoclave.
Dish washing
- Wash with dissolved powdered detergent. Rinse with DI water (Milli-Q if you're worried).
- Do not wash out water bottles with detergent!
- Do not wash empty 10% glycerol bottles with detergent!