Rao:Lab Tasks: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
{|
{|
=Autoclaving=
=Autoclaving=
*All dry items (tips, flasks, tubes, etc.) - 20 minutes steam, 20 minutes drying
*All liquids (plate agar, media, solutions) - 20 minutes steam
*All waste (old plates, etc.) - Must be in bio-hazard bag. 1 hr in big autoclave.
=Dish washing=
=Dish washing=
|}
*Wash with dissolved powdered detergent. Rinse with DI water (Milli-Q if you're worried).
*Do not wash out water bottles with detergent!
*Do not wash empty 10% glycerol bottles with detergent!

Latest revision as of 10:19, 30 November 2006

Autoclaving

  • All dry items (tips, flasks, tubes, etc.) - 20 minutes steam, 20 minutes drying
  • All liquids (plate agar, media, solutions) - 20 minutes steam
  • All waste (old plates, etc.) - Must be in bio-hazard bag. 1 hr in big autoclave.

Dish washing

  • Wash with dissolved powdered detergent. Rinse with DI water (Milli-Q if you're worried).
  • Do not wash out water bottles with detergent!
  • Do not wash empty 10% glycerol bottles with detergent!