Rao:Projects

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(Chemotaxis in Bacillus subtilis)
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==<center>'''Chemotaxis in ''Bacillus subtilis'''''</center>==
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==<center>'''''Bacillus subtilis'' Chemotaxis'''</center>==
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We are investigating chemotaxis in the model Gram-positive bacterium ''Bacillus subtilis''. While ''Escherichia coli'' has long been the paradigm for bacterial chemotaxis, the pathway architecture is not conserved in other species of bacteria. Interestingly, ''B. subtilis'' behaves in an identical manner to ''E. coli'' with regards to chemotaxis. The pathways in the two bacteria also have homologous proteins. Yet, how these proteins are “wired” to one another in the two pathways is entirely different. We seek to understand why these two pathways are “wired” differently and the extent of degeneracy in the basic design.
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<br>
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'''Attractant binding induces distinct structural changes to the polar and lateral signaling clusters in ''Bacillus subtilis'' chemotaxis.''' <br>
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''Journal of Biological Chemistry'', November 2010,  <br>
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Wu K, Walukiewicz HE, Glekas GD, Ordal GW, [[User:ChrisRao|Rao CV]] <br>
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[http://www.ncbi.nlm.nih.gov/pubmed/21098025 PubMed]
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<br>
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'''Site-specific methylation in ''Bacillus subtilis'' chemotaxis: The effect of covalent modifications to the chemotaxis receptor McpB.''' <br>
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''Microbiology'', September 2010,  <br>
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Glekas GD, Cates JR, Cohen TM, [[User:ChrisRao|Rao CV]], Ordal GW <br>
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[http://www.ncbi.nlm.nih.gov/pubmed/20864474 PubMed]
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<br>
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'''A PAS domain binds asparagine in the chemotaxis receptor McpB in ''Bacillus subtilis''''' <br>
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''Journal of Biological Chemistry'', 285(3):1870-8, 2010 <br>
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Glekas GD, Foster RM, Cates JR, Estrella JA, Wawrzyniak MJ, [[User:ChrisRao|Rao CV]], Ordal GW <br>
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[http://www.ncbi.nlm.nih.gov/pubmed/19864420 PubMed] [http://toothbase.scs.uiuc.edu/~chris/glekas_jbc2010.pdf PDF]
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<br>
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'''The molecular basis of excitation and adaptation during chemotactic sensory transduction in bacteria''' <br>
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''Contributions to Microbiology'', 16:33-64, 2009 <br>
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[[User:ChrisRao|Rao CV]], Ordal GW <br>
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[http://www.ncbi.nlm.nih.gov/pubmed/19494578 PubMed] [http://toothbase.scs.uiuc.edu/~chris/raoordal2009.pdf PDF]
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<br>
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'''The three adaptation systems of ''Bacillus subtilis chemotaxis''''' <br>
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''Trends in Microbiology'', 16(10):480-7, 2008 <br>
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[[User:ChrisRao|Rao CV]], Glekas GD, Ordal GW <br>
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[http://www.ncbi.nlm.nih.gov/pubmed/18774298 PubMed] [http://toothbase.scs.uiuc.edu/~chris/rao_trends2008.pdf PDF]
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<br>
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'''Phosphatase localization in bacterial chemotaxis: divergent mechanisms, convergent principles''' <br>
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''Physical Biology'', 14;2(3):148-58, 2005 <br>
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[[User:ChrisRao|Rao CV]], Kirby JR, Arkin AP <br>
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[http://www.ncbi.nlm.nih.gov/pubmed/16224120 PubMed] [http://toothbase.scs.uiuc.edu/~chris/rao2005.pdf PDF]
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<br>
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'''Design and diversity in bacterial chemotaxis: a comparative study in ''Escherichia coli'' and ''Bacillus subtilis''''' <br>
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''PLoS Biology'', 2(2):E49, 2004 <br>
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[[User:ChrisRao|Rao CV]], Kirby JR, Arkin AP <br>
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[http://www.ncbi.nlm.nih.gov/pubmed/14966542 PubMed] [http://toothbase.scs.uiuc.edu/~chris/rao_plos2004.pdf PDF]

Revision as of 20:21, 16 December 2010

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Bacillus subtilis Chemotaxis

We are investigating chemotaxis in the model Gram-positive bacterium Bacillus subtilis. While Escherichia coli has long been the paradigm for bacterial chemotaxis, the pathway architecture is not conserved in other species of bacteria. Interestingly, B. subtilis behaves in an identical manner to E. coli with regards to chemotaxis. The pathways in the two bacteria also have homologous proteins. Yet, how these proteins are “wired” to one another in the two pathways is entirely different. We seek to understand why these two pathways are “wired” differently and the extent of degeneracy in the basic design.


Attractant binding induces distinct structural changes to the polar and lateral signaling clusters in Bacillus subtilis chemotaxis.
Journal of Biological Chemistry, November 2010,
Wu K, Walukiewicz HE, Glekas GD, Ordal GW, Rao CV
PubMed


Site-specific methylation in Bacillus subtilis chemotaxis: The effect of covalent modifications to the chemotaxis receptor McpB.
Microbiology, September 2010,
Glekas GD, Cates JR, Cohen TM, Rao CV, Ordal GW
PubMed


A PAS domain binds asparagine in the chemotaxis receptor McpB in Bacillus subtilis
Journal of Biological Chemistry, 285(3):1870-8, 2010
Glekas GD, Foster RM, Cates JR, Estrella JA, Wawrzyniak MJ, Rao CV, Ordal GW
PubMed PDF


The molecular basis of excitation and adaptation during chemotactic sensory transduction in bacteria
Contributions to Microbiology, 16:33-64, 2009
Rao CV, Ordal GW
PubMed PDF


The three adaptation systems of Bacillus subtilis chemotaxis
Trends in Microbiology, 16(10):480-7, 2008
Rao CV, Glekas GD, Ordal GW
PubMed PDF


Phosphatase localization in bacterial chemotaxis: divergent mechanisms, convergent principles
Physical Biology, 14;2(3):148-58, 2005
Rao CV, Kirby JR, Arkin AP
PubMed PDF


Design and diversity in bacterial chemotaxis: a comparative study in Escherichia coli and Bacillus subtilis
PLoS Biology, 2(2):E49, 2004
Rao CV, Kirby JR, Arkin AP
PubMed PDF

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