Rao:Protocols: Difference between revisions
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< | __NOTOC__ | ||
==<center>General Techniques</center>== | |||
*[[Rao:ColPCR | Colony PCR Verification]] | *[[Rao:ColPCR | Colony PCR Verification]] | ||
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]] | *[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]] | ||
*[[Rao:Mega-Primer | Mega-Primer]] | *[[Rao:Mega-Primer | Mega-Primer]] | ||
*[[Rao:Restriction_Digest | Restriction Digest]] | *[[Rao:Restriction_Digest | Restriction Digest]] | ||
*[[Rao:Ligation | DNA Ligation]] | *[[Rao:Ligation | DNA Ligation]] | ||
*[[Rao:BetaGal | Beta-galactosidase Assay]] | |||
*[[Rao:Tecan | Tecan Safire2]] | |||
==<center>''E. coli/S. typhimurium'' specific</center>== | |||
*[[Rao:Transformation | DNA Transformation]] | *[[Rao:Transformation | DNA Transformation]] | ||
*[[Rao: | *[[Rao:Transduction | Transduction]] | ||
*[[Rao:WannerKO | One-step Gene Distruption]] | *[[Rao:WannerKO | One-step Gene Distruption]] | ||
*[[Rao:CRIM | CRIM Plasmid Integration]] | |||
==<center>''B. subtilis specific''</center>== | |||
*[[Rao:BsTransformation | B. subtilis transformation]] | *[[Rao:BsTransformation | B. subtilis transformation]] | ||
==<center>Media Preparation</center>== | |||
< | |||
*[[Rao:LB | LB]] | *[[Rao:LB | LB]] | ||
*[[Rao:SOBC | SOB/SOC]] | *[[Rao:SOBC | SOB/SOC]] | ||
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*[[Rao:Penassay | Penassay Broth]] | *[[Rao:Penassay | Penassay Broth]] | ||
*[[Rao:MotilityPlates | Motility Plates]] | *[[Rao:MotilityPlates | Motility Plates]] | ||
If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here] | |||
Latest revision as of 19:00, 16 December 2010
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Rao Lab
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