Rao:Protocols

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<h4>PCR</h4>
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__NOTOC__
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==<center>General Techniques</center>==
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*[[Rao:ColPCR | Colony PCR Verification]]
*[[Rao:ColPCR | Colony PCR Verification]]
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]]
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]]
*[[Rao:Mega-Primer | Mega-Primer]]
*[[Rao:Mega-Primer | Mega-Primer]]
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<h4>Need-to-know Techniques</h4>
 
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'''''General'''''
 
*[[Rao:Restriction_Digest | Restriction Digest]]
*[[Rao:Restriction_Digest | Restriction Digest]]
*[[Rao:Ligation | DNA Ligation]]
*[[Rao:Ligation | DNA Ligation]]
*[[Rao:BetaGal | Beta-galactosidase Assay]]
*[[Rao:BetaGal | Beta-galactosidase Assay]]
*[[Rao:Tecan | Tecan Safire2]]
*[[Rao:Tecan | Tecan Safire2]]
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'''''E. coli/S. typhimurium specific'''''
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==<center>''E. coli/S. typhimurium'' specific</center>==
*[[Rao:Transformation | DNA Transformation]]
*[[Rao:Transformation | DNA Transformation]]
*[[Rao:Transduction | Transduction]]
*[[Rao:Transduction | Transduction]]
*[[Rao:WannerKO | One-step Gene Distruption]]
*[[Rao:WannerKO | One-step Gene Distruption]]
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*[[Rao:CRIM | CRIM Plasmid Integration]]
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'''''B. subtilis specific'''''
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==<center>''B. subtilis specific''</center>==
*[[Rao:BsTransformation | B. subtilis transformation]]
*[[Rao:BsTransformation | B. subtilis transformation]]
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==<center>Media Preparation</center>==
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<h4>Media Preparation</h4>
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*[[Rao:LB | LB]]
*[[Rao:LB | LB]]
*[[Rao:SOBC | SOB/SOC]]
*[[Rao:SOBC | SOB/SOC]]
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*[[Rao:Penassay | Penassay Broth]]
*[[Rao:Penassay | Penassay Broth]]
*[[Rao:MotilityPlates | Motility Plates]]
*[[Rao:MotilityPlates | Motility Plates]]
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If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html#a9 here]
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If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here]
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[[Rao_Lab | Back to Rao Lab]]
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General Techniques

E. coli/S. typhimurium specific

B. subtilis specific

Media Preparation

If your interested in more recipes go here

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