Rao:Protocols
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| - | < | + | __NOTOC__ |
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| + | ==<center>General Techniques</center>== | ||
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| + | *[[Rao:ColPCR | Colony PCR Verification]] | ||
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]] | *[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]] | ||
*[[Rao:Mega-Primer | Mega-Primer]] | *[[Rao:Mega-Primer | Mega-Primer]] | ||
| - | + | *[[Rao:Restriction_Digest | Restriction Digest]] | |
| - | | | + | *[[Rao:Ligation | DNA Ligation]] |
| - | + | *[[Rao:BetaGal | Beta-galactosidase Assay]] | |
| - | |- | + | *[[Rao:Tecan | Tecan Safire2]] |
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| - | + | ==<center>''E. coli/S. typhimurium'' specific</center>== | |
| - | + | *[[Rao:Transformation | DNA Transformation]] | |
| - | *[[Rao: | + | *[[Rao:Transduction | Transduction]] |
| - | *[[Rao: | + | *[[Rao:WannerKO | One-step Gene Distruption]] |
| - | </ | + | *[[Rao:CRIM | CRIM Plasmid Integration]] |
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| - | + | ==<center>''B. subtilis specific''</center>== | |
| - | + | *[[Rao:BsTransformation | B. subtilis transformation]] | |
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| - | + | ==<center>Media Preparation</center>== | |
| - | + | *[[Rao:LB | LB]] | |
| - | *[[Rao: | + | *[[Rao:SOBC | SOB/SOC]] |
| - | *[[Rao: | + | *[[Rao:Min9 | M9/M9c ]] |
| - | + | *[[Rao:TB | Tryptone Broth (TB)]] | |
| - | | | + | *[[Rao:Penassay | Penassay Broth]] |
| + | *[[Rao:MotilityPlates | Motility Plates]] | ||
| + | If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here] | ||
Current revision
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Rao Lab
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