Rao:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (22:00, 16 December 2010) (view source)
 
(21 intermediate revisions not shown.)
Line 1: Line 1:
-
{| cellspacing="3"  
+
{{Rao}}
-
|- valign="top"
+
{|cellspacing="5" cellpadding="10" style="background:#3674C2; width: 750px;"
-
|width="300" class="MainPageBG" style="border: 1px solid #00007B; color: #000; background-color: #fff3f3"|
+
|-valign="top"
-
<div style="padding: .4em .9em .9em">
+
|style="background:#ffffff"|
-
<h4>PCR</h4>
+
__NOTOC__
 +
 
 +
==<center>General Techniques</center>==
 +
 
 +
*[[Rao:ColPCR | Colony PCR Verification]]
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]]
*[[Rao:EI-PCR | Enzymatic Inverse PCR (EI-PCR)]]
*[[Rao:Mega-Primer | Mega-Primer]]
*[[Rao:Mega-Primer | Mega-Primer]]
-
</div>
+
*[[Rao:Restriction_Digest | Restriction Digest]]
-
|}
+
*[[Rao:Ligation | DNA Ligation]]
 +
*[[Rao:BetaGal | Beta-galactosidase Assay]]
 +
*[[Rao:Tecan | Tecan Safire2]]
 +
 
 +
==<center>''E. coli/S. typhimurium'' specific</center>==
 +
*[[Rao:Transformation | DNA Transformation]]
 +
*[[Rao:Transduction | Transduction]]
 +
*[[Rao:WannerKO | One-step Gene Distruption]]
 +
*[[Rao:CRIM | CRIM Plasmid Integration]]
 +
 
 +
==<center>''B. subtilis specific''</center>==
 +
*[[Rao:BsTransformation | B. subtilis transformation]]
 +
 
 +
==<center>Media Preparation</center>==
 +
*[[Rao:LB | LB]]
 +
*[[Rao:SOBC | SOB/SOC]]
 +
*[[Rao:Min9 | M9/M9c ]]
 +
*[[Rao:TB | Tryptone Broth (TB)]]
 +
*[[Rao:Penassay | Penassay Broth]]
 +
*[[Rao:MotilityPlates | Motility Plates]]
 +
If your interested in more recipes go [http://molbiol.edu.ru/eng/protocol/01_06.html here]

Current revision

Rao Lab

Home        Members        Projects        Publications        Protocols        Code        Contact       



General Techniques

E. coli/S. typhimurium specific

B. subtilis specific

Media Preparation

If your interested in more recipes go here

Personal tools