Rao:Protocols

PCR

• Colony PCR Verification
• Enzymatic Inverse PCR (EI-PCR)
• Mega-Primer

Classic Techniques

General techniques

• Beta-galactosidase Assay
• Restriction Digest
• DNA Ligation

E. coli/S. typhimurium specific

• DNA Transformation
• Transduction
• One-step Gene Distruption

B. subtilis specific

• B. subtilis transformation

Media Preparation

• LB
• SOB/SOC
• M9/M9c
• Tryptone Broth (TB)
• Penassay Broth
• Motility Plates