Rapid Mouse Activity Assay: Difference between revisions
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==Procedure== | ==Procedure== | ||
The activity assay should be done always at the same period of the day. | The activity assay should be done always at the same period of the day. | ||
*1. Prepare clean cage: spray with 10% Bleach and dry with paper towel | |||
*2. Place mouse into the clean cage that is divided into 4 quadrants (Place cage on top of a piece of paper with 4 quadrants drawn) | |||
*3. Cover the cage with regular plastic cage lid | |||
*4. No measurements on the 1st minute (acclimation) | |||
*5. Next 3 minutes: Score the activity | |||
Two or more feet into new quadrant = 1 point | |||
Rising up on hind paws (to explore wall or top of cage) = 2 points | |||
Tests are done in the late morning. In our experiments with FVB/N mice the average for control mice is 80 ± 19. | Tests are done in the late morning. In our experiments with FVB/N mice the average for control mice is 80 ± 19. | ||
When mice get sick from cardiomyopathy they score less than 10. If they score less than 10 on two consecutive days we sacrifice these mice, or "recover" them since they invariably (>95%) die in the following days if no intervention is taken. | When mice get sick from cardiomyopathy they score less than 10. If they score less than 10 on two consecutive days we sacrifice these mice, or "recover" them since they invariably (>95%) die in the following days if no intervention is taken. | ||
==Notes== | ==Notes== | ||
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'''Relevant papers and books''' | '''Relevant papers and books''' | ||
No further references are available at this time | |||
==Contact== | ==Contact== | ||
Latest revision as of 15:31, 24 June 2009
Overview
List of reagents is not yet complete
Procedure
The activity assay should be done always at the same period of the day.
- 1. Prepare clean cage: spray with 10% Bleach and dry with paper towel
- 2. Place mouse into the clean cage that is divided into 4 quadrants (Place cage on top of a piece of paper with 4 quadrants drawn)
- 3. Cover the cage with regular plastic cage lid
- 4. No measurements on the 1st minute (acclimation)
- 5. Next 3 minutes: Score the activity
Two or more feet into new quadrant = 1 point
Rising up on hind paws (to explore wall or top of cage) = 2 points
Tests are done in the late morning. In our experiments with FVB/N mice the average for control mice is 80 ± 19.
When mice get sick from cardiomyopathy they score less than 10. If they score less than 10 on two consecutive days we sacrifice these mice, or "recover" them since they invariably (>95%) die in the following days if no intervention is taken.
Notes
No further notes are available at this time.
References
Relevant papers and books
No further references are available at this time
Contact
- Who has experience with this protocol?
or instead, discuss this protocol.
