Reagents for SPR: Difference between revisions
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|purpose|| | |reagents||purpose||M<sub>r</sub>||handling instructions | ||
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|amine coupling | |1-ethyl-3-(3-dimethyl | ||
|N-hydroxysuccinimide (NHS)<br> | aminopropyl) carbodiimide ('''EDC''')<br> | ||
ethanolamine | |||
|[[Amine coupling method in SPR|amine coupling]] | |||
|191.70 | |||
|EDC stock solution 0.4M<br> | |||
resuspend fresh ultrapure reagent in milli-Q water<br> | |||
aliquote into 200μL portions directly into vials to be used on the instrument<br> | |||
freeze the aliquotes and store at -20 for no more then 2 months<br> | |||
if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 10 mL water | |||
|- | |||
|N-hydroxysuccinimide ('''NHS''')<br> | |||
|[[Amine coupling method in SPR|amine coupling]] | |||
|115.09 | |||
|NHS stock solution 0.1M<br> | |||
prepare and store as desribed for EDC<br> | |||
Biacore supplied 115 mg portion should be dissolved in 10 mL water | |||
|} | |||
[[Category:SPR]] | [[Category:SPR]] | ||
Latest revision as of 14:23, 19 May 2008
| reagents | purpose | Mr | handling instructions |
| 1-ethyl-3-(3-dimethyl
aminopropyl) carbodiimide (EDC) |
amine coupling | 191.70 | EDC stock solution 0.4M resuspend fresh ultrapure reagent in milli-Q water |
| N-hydroxysuccinimide (NHS) |
amine coupling | 115.09 | NHS stock solution 0.1M prepare and store as desribed for EDC |
