Reagents for SPR

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|N-hydroxysuccinimide ('''NHS''')<br>
|N-hydroxysuccinimide ('''NHS''')<br>
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|[[Amine coupling method in SPR|amine coupling]]
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[[Category:SPR]]
[[Category:SPR]]

Revision as of 16:44, 19 May 2008

reagentspurposeMrhandling instructions
1-ethyl-3-(3-dimethyl

aminopropyl) carbodiimide (EDC)
ethanolamine

amine coupling 191.70 EDC stock solution 0.4M

resuspend fresh ultrapure reagent in milli-Q water
aliquote into 150μL portions directly into vials to be used on the instrument
freeze the aliquotes and store at -20 for no more then 2 months

N-hydroxysuccinimide (NHS)
amine coupling
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