Reagents for SPR

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|N-hydroxysuccinimide ('''NHS''')<br>
|N-hydroxysuccinimide ('''NHS''')<br>
|[[Amine coupling method in SPR|amine coupling]]
|[[Amine coupling method in SPR|amine coupling]]
-
 
+
|115.09
 +
|NHS stock solution 0.1M<br>
 +
prepare and store as desribed for EDC
|}
|}
[[Category:SPR]]
[[Category:SPR]]

Revision as of 16:46, 19 May 2008

reagentspurposeMrhandling instructions
1-ethyl-3-(3-dimethyl

aminopropyl) carbodiimide (EDC)
ethanolamine

amine coupling 191.70 EDC stock solution 0.4M

resuspend fresh ultrapure reagent in milli-Q water
aliquote into 150μL portions directly into vials to be used on the instrument
freeze the aliquotes and store at -20 for no more then 2 months

N-hydroxysuccinimide (NHS)
amine coupling 115.09 NHS stock solution 0.1M

prepare and store as desribed for EDC

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