Reagents for SPR
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(Difference between revisions)
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|EDC stock solution 0.4M<br> | |EDC stock solution 0.4M<br> | ||
resuspend fresh ultrapure reagent in milli-Q water<br> | resuspend fresh ultrapure reagent in milli-Q water<br> | ||
| - | aliquote into | + | aliquote into 200μL portions directly into vials to be used on the instrument<br> |
freeze the aliquotes and store at -20 for no more then 2 months | freeze the aliquotes and store at -20 for no more then 2 months | ||
|- | |- | ||
Revision as of 17:02, 19 May 2008
| reagents | purpose | Mr | handling instructions |
| 1-ethyl-3-(3-dimethyl
aminopropyl) carbodiimide (EDC) | amine coupling | 191.70 | EDC stock solution 0.4M resuspend fresh ultrapure reagent in milli-Q water |
| N-hydroxysuccinimide (NHS) | amine coupling | 115.09 | NHS stock solution 0.1M prepare and store as desribed for EDC |
