Reagents for SPR

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 12: Line 12:
aliquote into 200&mu;L portions directly into vials to be used on the instrument<br>  
aliquote into 200&mu;L portions directly into vials to be used on the instrument<br>  
freeze the aliquotes and store at -20 for no more then 2 months<br>
freeze the aliquotes and store at -20 for no more then 2 months<br>
-
if you buy EDC from Biacore - suspend 0.75 g portion supplied in 9.781 mL water
+
if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 9.781 mL water
|-
|-
|N-hydroxysuccinimide ('''NHS''')<br>
|N-hydroxysuccinimide ('''NHS''')<br>
Line 18: Line 18:
|115.09
|115.09
|NHS stock solution 0.1M<br>
|NHS stock solution 0.1M<br>
-
prepare and store as desribed for EDC
+
prepare and store as desribed for EDC<br>
 +
Biacore supplied 115 mg portion should be dissolved in 10 mL water
|}
|}
[[Category:SPR]]
[[Category:SPR]]

Revision as of 16:05, 19 May 2008

reagentspurposeMrhandling instructions
1-ethyl-3-(3-dimethyl

aminopropyl) carbodiimide (EDC)
ethanolamine

amine coupling 191.70 EDC stock solution 0.4M

resuspend fresh ultrapure reagent in milli-Q water
aliquote into 200μL portions directly into vials to be used on the instrument
freeze the aliquotes and store at -20 for no more then 2 months
if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 9.781 mL water

N-hydroxysuccinimide (NHS)
amine coupling 115.09 NHS stock solution 0.1M

prepare and store as desribed for EDC
Biacore supplied 115 mg portion should be dissolved in 10 mL water

Personal tools