Reagents for SPR

reagents	purpose	Mr	handling instructions
1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) ethanolamine	amine coupling	191.70	EDC stock solution 0.4M resuspend fresh ultrapure reagent in milli-Q water aliquote into 200μL portions directly into vials to be used on the instrument freeze the aliquotes and store at -20 for no more then 2 months if you buy EDC from Biacore - dissolve 0.75 g portion supplied in 10 mL water
N-hydroxysuccinimide (NHS)	amine coupling	115.09	NHS stock solution 0.1M prepare and store as desribed for EDC Biacore supplied 115 mg portion should be dissolved in 10 mL water