Recombineering: Difference between revisions
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<math>\lambda</math> phage exo, bet, and gam genes are integrated into E-coli to demonstrate phage promoted homologous recombination. | <math>\lambda</math> phage exo, bet, and gam genes are integrated into E-coli to demonstrate phage promoted homologous recombination. | ||
[http://jb.asm.org/cgi/gca?sendit=Get+All+Checked+Abstract%28s%29&SEARCHID=1&VOLUME=180&FIRSTPAGE=2063&FIRSTINDEX=0&hits=10&RESULTFORMAT=&gca=jb%3B180%2F8%2F2063 Murphy 1998] <math>lambda</math> Red-Promoted Gene Replacement | [http://jb.asm.org/cgi/gca?sendit=Get+All+Checked+Abstract%28s%29&SEARCHID=1&VOLUME=180&FIRSTPAGE=2063&FIRSTINDEX=0&hits=10&RESULTFORMAT=&gca=jb%3B180%2F8%2F2063 Murphy 1998] <math>\lambda</math> Red-Promoted Gene Replacement | ||
Revision as of 15:56, 27 April 2006
Recombineering is a method where E-coli undergoes recombination between linear DNA, introduced through electroporation, with circularized DNA already present within the cell.
http://recombineering.ncifcrf.gov/
[math]\displaystyle{ \lambda }[/math] phage exo, bet, and gam genes are integrated into E-coli to demonstrate phage promoted homologous recombination.
Murphy 1998 [math]\displaystyle{ \lambda }[/math] Red-Promoted Gene Replacement
To acheive control over phage recombination gene activity, temperature sensitive [math]\displaystyle{ \lambda }[/math] repressor (cI857) was introduced.
Yu 2000 An efficient recombination system for chromosome engineering in Escherichia coli
This process is then adapted to E-coli strain DH10B, a Bacteria Artificial Chromosome (BAC)strain. This results in the original recombineering E-coli strain designated DY380 as well as other selection modified versions.
Lee 2001. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.
