Recombineering
Recombineering is a method where E-coli undergoes recombination between linear DNA, introduced through electroporation, with circularized DNA already present within the cell.
http://recombineering.ncifcrf.gov/
[math]\displaystyle{ \lambda }[/math] phage exo, bet, and gam genes are integrated into E-coli to demonstrate phage promoted homologous recombination.
Murphy 1998 [math]\displaystyle{ \lambda }[/math] Red-Promoted Gene Replacement
To acheive control over phage recombination gene activity, temperature sensitive [math]\displaystyle{ \lambda }[/math] repressor (cI857) was introduced.
Yu 2000 An efficient recombination system for chromosome engineering in Escherichia coli
This process is then adapted to E-coli strain DH10B, a Bacteria Artificial Chromosome (BAC)strain. This results in the original recombineering E-coli strain designated DY380 as well as other versions modified to include different selection mechanisms.
Lee 2001. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA
All about the [math]\displaystyle{ \lambda }[/math] phage recombination proteins!!
Little 1967 An Exonuclease Induced by phage [math]\displaystyle{ \lambda\lt \math\gt [http://www.jbc.org/cgi/content/abstract/256/24/12636 Kmiec 1981]\lt math\gt \beta\lt \math\gt protein of phage \lt math\gt \lambda\lt \math\gt promotes renaturation of DNA [http://www.jbc.org/cgi/content/abstract/261/16/7472 Muniyappa 1986] Pairing activities of \lt math\gt \beta\lt \math\gt protein in phage \lt math\gt \lambda\lt \math\gt . [http://www.jbc.org/cgi/content/abstract/250/18/7377 Karu 1975] How \lt math\gt \gamma\lt \math\gt protein specified by phage \lt math\gt \lambda\lt \math\gt affects recBC enzyme in E. Coli [http://jb.asm.org/cgi/gca?sendit=Get+All+Checked+Abstract%28s%29&SEARCHID=1&VOLUME=173&FIRSTPAGE=5808&FIRSTINDEX=0&hits=10&RESULTFORMAT=&gca=jb%3B173%2F18%2F5808 Murphy 1991] \lt math\gt \gamma\lt \math\gt protein of phage \lt math\gt \lambda\lt \math\gt inhibits recombination activites of RecBCD enzyme. [http://www.jbc.org/cgi/content/abstract/246/8/2502 Carter 1971] Roles of Exonuclease and \lt math\gt \beta\lt \math\gt protein of phage \lt math\gt \lambda\lt \math\gt in recombination }[/math]
