References and Papers: Difference between revisions
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===Functional Assays=== | ===Functional Assays=== | ||
'''Cellulase''' | '''Cellulase''' | ||
We're most interested in endo-1,4-beta-glucanases, as they are the rate limiting step in cellulose degradation. However, it may be necessary to include exocellulases to relieve product inhibition (this may or may not be true, see: doi:10.1016/0076-6879(88)60109-1 <--- suggests there is no synergism between exo's and endo's.... | We're most interested in endo-1,4-beta-glucanases, as they are the rate limiting step in cellulose degradation. However, it may be necessary to include exocellulases to relieve product inhibition (this may or may not be true, see: doi:10.1016/0076-6879(88)60109-1 <--- suggests there is no synergism between exo's and endo's.... | ||
This is the sequencing paper from which we have taken all of our (Cellvibrio japonicus) "cellulases" up to this point: doi:10.1128/JB.01701-07 | |||
This is a Carboxymethylcellulose (CMC) assay (CMC is soluble) that is colorimetric: | This is a Carboxymethylcellulose (CMC) assay (CMC is soluble) that is colorimetric: | ||
Revision as of 15:40, 26 June 2009
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University of California Berkeley iGem 2009 Wetlab Team |
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Links
Post brief discussions of and links to interesting papers and references.
Functional Assays
Cellulase We're most interested in endo-1,4-beta-glucanases, as they are the rate limiting step in cellulose degradation. However, it may be necessary to include exocellulases to relieve product inhibition (this may or may not be true, see: doi:10.1016/0076-6879(88)60109-1 <--- suggests there is no synergism between exo's and endo's....
This is the sequencing paper from which we have taken all of our (Cellvibrio japonicus) "cellulases" up to this point: doi:10.1128/JB.01701-07
This is a Carboxymethylcellulose (CMC) assay (CMC is soluble) that is colorimetric: http://secure.megazyme.com/downloads/en/data/S-ACMC.pdf
Here is an example of a cellulase being displayed by an Ice-Nucleation Protein displayer: doi:10.1016/S0141-0229(97)00224-X (of particular note is the congo-red plate-based assay)
Superoxide dismutase
