Refolding Proteins

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==Resources==
==Resources==
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*[[Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding]] - Qiagen Ni NTA spin columns
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*[[Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding]] - Small scale (up to 0.5 to 1 mg protein, from 50mL culture) - Qiagen Ni NTA spin columns
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*[[Sauer:Purification_of_His-tagged_proteins/Denaturing_prep]] - Qiagen Ni NTA '''resin''' (goo).
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*[[Sauer:Purification_of_His-tagged_proteins/Denaturing_prep]] - Large scale (1 L culture) - Qiagen Ni NTA '''resin''' (goo).
*[http://refold.med.monash.edu.au/ REFOLD] - Repository of refolding protocols for specific protocols.
*[http://refold.med.monash.edu.au/ REFOLD] - Repository of refolding protocols for specific protocols.

Revision as of 14:18, 14 August 2008

Introduction

Many recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations demanded by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify denatured protein from these inclusion bodies and then refold them manually.

Resources

See also

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