Refolding Proteins

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(Introduction)
Current revision (14:22, 14 August 2008) (view source)
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==Introduction==
==Introduction==
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Denaturing and then refolding proteins can be a good way to increase yield, because purification in denaturing conditions reduces nonspecific binding.
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Denaturing and then refolding proteins can be a good way to increase yield, because purification in denaturing conditions reduces nonspecific binding. You do, however, risk misfolding the protein.
In other cases, recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations desired by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify the denatured protein from these inclusion bodies and then refold them manually.
In other cases, recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations desired by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify the denatured protein from these inclusion bodies and then refold them manually.

Current revision

Introduction

Denaturing and then refolding proteins can be a good way to increase yield, because purification in denaturing conditions reduces nonspecific binding. You do, however, risk misfolding the protein.

In other cases, recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations desired by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify the denatured protein from these inclusion bodies and then refold them manually.

Please note that the below protocols do not involve purification from inclusion bodies. To do so, you can add lysozyme to reach 1 mM to the cell pellet, then vortex for 1 minute.

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See also

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