Refolding Proteins: Difference between revisions
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==Resources== | ==Resources== | ||
*[[Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding]] | *[[Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding]] - Qiagen Ni NTA spin columns | ||
*[[Sauer:Purification_of_His-tagged_proteins/Denaturing_prep]] | *[[Sauer:Purification_of_His-tagged_proteins/Denaturing_prep]] - Qiagen Ni NTA '''resin''' (goo). | ||
*[http://refold.med.monash.edu.au/ REFOLD] - Repository of refolding protocols for specific protocols. | *[http://refold.med.monash.edu.au/ REFOLD] - Repository of refolding protocols for specific protocols. | ||
Revision as of 10:49, 12 August 2008
Introduction
Many recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations demanded by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify denatured protein from these inclusion bodies and then refold them manually.
Resources
- Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding - Qiagen Ni NTA spin columns
- Sauer:Purification_of_His-tagged_proteins/Denaturing_prep - Qiagen Ni NTA resin (goo).
- REFOLD - Repository of refolding protocols for specific protocols.
See also
- IGEM:IMPERIAL/2007/CFS - Cell free systems for protein expression.
