Refolding Proteins
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==Resources== | ==Resources== | ||
| - | *[[Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding]] - Qiagen Ni NTA spin columns | + | *[[Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding]] - Small scale (up to 0.5 to 1 mg protein, from 50mL culture) - Qiagen Ni NTA spin columns |
| - | *[[Sauer:Purification_of_His-tagged_proteins/Denaturing_prep]] - Qiagen Ni NTA '''resin''' (goo). | + | *[[Sauer:Purification_of_His-tagged_proteins/Denaturing_prep]] - Large scale (1 L culture) - Qiagen Ni NTA '''resin''' (goo). |
*[http://refold.med.monash.edu.au/ REFOLD] - Repository of refolding protocols for specific protocols. | *[http://refold.med.monash.edu.au/ REFOLD] - Repository of refolding protocols for specific protocols. | ||
Revision as of 15:18, 14 August 2008
Introduction
Many recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations demanded by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify denatured protein from these inclusion bodies and then refold them manually.
Resources
- Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding - Small scale (up to 0.5 to 1 mg protein, from 50mL culture) - Qiagen Ni NTA spin columns
- Sauer:Purification_of_His-tagged_proteins/Denaturing_prep - Large scale (1 L culture) - Qiagen Ni NTA resin (goo).
- REFOLD - Repository of refolding protocols for specific protocols.
See also
- IGEM:IMPERIAL/2007/CFS - Cell free systems for protein expression.
