Refolding Proteins

Introduction

Many recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations demanded by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify denatured protein from these inclusion bodies and then refold them manually.

Resources

• Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding - Qiagen Ni NTA spin columns
• Sauer:Purification_of_His-tagged_proteins/Denaturing_prep - Qiagen Ni NTA resin (goo).
• REFOLD - Repository of refolding protocols for specific protocols.

See also

• IGEM:IMPERIAL/2007/CFS - Cell free systems for protein expression.