Many recombinant or fusion proteins may be unfavorable to host bacteria, especially at the concentrations demanded by researchers. As a result, these proteins may be misfolded and segregated into inclusion bodies. It may be necessary to purify denatured protein from these inclusion bodies and then refold them manually.
- Knight:Purification_of_His-tagged_proteins/Denaturing_with_refolding - Small scale (up to 0.5 to 1 mg protein, from 50mL culture) - Qiagen Ni NTA spin columns
- Sauer:Purification_of_His-tagged_proteins/Denaturing_prep - Large scale (1 L culture) - Qiagen Ni NTA resin (goo).
- REFOLD - Repository of refolding protocols for specific protocols.
- IGEM:IMPERIAL/2007/CFS - Cell free systems for protein expression.