Registry/Measurement kit: Difference between revisions

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The Measurement Kit consists of Promoter Testers, RBS Testers, and an Inverter/Terminator Tester. All Devices are on the 3K3 plasmid.


==Project Pages==
*[[The BioBricks Foundation:Standards/Technical/Measurement]] - multi-institution comparison of promoter set.
*[[Registry/Measurement kit/Notebook|Current notebook]]
**[[Registry/Measurement kit/Instructions]]
**[[Registry/Measurement kit/Preparation]]
**[[Registry/Measurement kit/RBS Measurement Kit]]
**[[Registry/Measurement kit/Promoter Measurement Kit]]
*Two summer notebooks on construction of the kit:[[Registry/Measurement kit/Notebook|Nishant]], [[User:Jessica_Karen_Wong/Notebook|Karen]]
*[[Registry/Measurement kit/Notebook/Design|Some early design notes]] ([[User:Jessica Karen Wong/Design|Karen's design notes]])
*[[User:Jessica Karen Wong/Construction|Construction approach]] ([[User:Jessica Karen Wong/Primers|Primers used in construction]])
*[[Registry/Measurement kit/Glycerol box|Glycerol box]]
*[[Registry/Measurement kit/Previous versions]]


==Promoter Tester==
==References==
The Promoter Tester using GFP is based on <BBpart> E0240</BBpart> and using RFP is based on <BBpart> I2057</BBpart>. Both come in two versions.
<biblio>
*Version 1 (E/S)
#Alper-PNAS-2005 pmid=16123130
**___E CCDB S___RBS GFP T
#Zaslaver-PNAS-2006 pmid=16862137
**Cut promoter E/S and insert
#Chen-NAR-1994 pmid=7528374
**Can take part back out if necessary
</biblio>
 
*Version 2
**___E CCDB X___E0240
**Cut Promoter E/S to insert
**Forms mixed site so can't remove, real conditions
 
==RBS Tester==
The RBS Tester using GFP is based on <BBpart> I2055</BBpart> and using RFP is based on <BBpart> I2056</BBpart>. Both come in two versions.
*Versions 1 (E/X)
**__R0040___E CCDB X____GFP T
**To insert RBS cut E/S, forms a mixed Spe/Xba site
**Has too big spacing (full BB prefix) b/t promoter and RBS
**Easier for testers to use
 
*Version 2 (S/X)
**__R0040___S CCDB X____GFP T
**Will get 2 spe/xba mixed sites, can't get RBS out
**Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
**Real condition spacing
 
==Inverter/Terminator Tester==
*Based on <BBpart> T9002</BBpart>
 
*F2620________E X P1010 S P________E0040
*Contains a full BB site to allow construction within the device

Latest revision as of 15:53, 22 March 2008

Project Pages

References

  1. Alper H, Fischer C, Nevoigt E, and Stephanopoulos G. Tuning genetic control through promoter engineering. Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12678-83. DOI:10.1073/pnas.0504604102 | PubMed ID:16123130 | HubMed [Alper-PNAS-2005]
  2. Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, and Alon U. A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods. 2006 Aug;3(8):623-8. DOI:10.1038/nmeth895 | PubMed ID:16862137 | HubMed [Zaslaver-PNAS-2006]
  3. Chen H, Bjerknes M, Kumar R, and Jay E. Determination of the optimal aligned spacing between the Shine-Dalgarno sequence and the translation initiation codon of Escherichia coli mRNAs. Nucleic Acids Res. 1994 Nov 25;22(23):4953-7. DOI:10.1093/nar/22.23.4953 | PubMed ID:7528374 | HubMed [Chen-NAR-1994]

All Medline abstracts: PubMed | HubMed

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