Registry/Measurement kit: Difference between revisions

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The Measurement Kit consists of Promoter Testers, RBS Testers, and an Inverter/Terminator Tester. All Devices are on the [http://parts.mit.edu/registry/index.php/Part:pSB3K3 3K3] plasmid.  Also, they are being built in both GFP and RFP versions.  (GFP versions shown below)


 
==Project Pages==
==Promoter Tester==
*[[The BioBricks Foundation:Standards/Technical/Measurement]] - multi-institution comparison of promoter set.
 
*[[Registry/Measurement kit/Notebook|Current notebook]]
[[Image:E0240.1.jpg|thumb|left|E0240 Version 1 Design]]
**[[Registry/Measurement kit/Instructions]]
The Promoter Tester using GFP is based on <BBpart> E0240</BBpart> and using RFP is based on <BBpart> I2057</BBpart>. Both come in two versions.
**[[Registry/Measurement kit/Preparation]]
*Version 1 (E/S)
**[[Registry/Measurement kit/RBS Measurement Kit]]
**___E X CCDB S___RBS GFP T
**[[Registry/Measurement kit/Promoter Measurement Kit]]
**Cut promoter E/S and insert
*Two summer notebooks on construction of the kit:[[Registry/Measurement kit/Notebook|Nishant]], [[User:Jessica_Karen_Wong/Notebook|Karen]]
**Can take part back out if necessary
*[[Registry/Measurement kit/Notebook/Design|Some early design notes]] ([[User:Jessica Karen Wong/Design|Karen's design notes]])
 
*[[User:Jessica Karen Wong/Construction|Construction approach]] ([[User:Jessica Karen Wong/Primers|Primers used in construction]])
*Version 2
*[[Registry/Measurement kit/Glycerol box|Glycerol box]]
**___E CCDB X___E0240
*[[Registry/Measurement kit/Previous versions]]
**Cut Promoter E/S to insert
**Forms mixed site so can't remove, real conditions
 
*GFP Promoter Tester Version 1 E/S <BBpart> I20244</BBpart>
*GFP Promoter Tester Version 2 E/X <BBpart> I20245</BBpart>
*RFP Promoter Tester Version 1 E/S <BBpart> I20246</BBpart>
*RFP Promoter Tester Version 2 E/X <BBpart> I20247</BBpart>
 
==RBS Tester==
[[Image:I2055.1.jpg|thumb|left|I2055  Design]]
The RBS Tester using GFP is based on <BBpart> I2055</BBpart> and using RFP is based on <BBpart> I2056</BBpart>. Both come in two versions.
*Versions 1 (E/X)
**__R0040___E CCDB X____GFP T
**To insert RBS cut E/S, forms a mixed Spe/Xba site
**Has too big spacing (full BB prefix) b/t promoter and RBS
**Easier for testers to use
 
*Version 2 (S/X)
**__R0040___S CCDB X____GFP T
**Will get 2 spe/xba mixed sites, can't get RBS out
**Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
**Real condition spacing
 
*GFP RBS Tester Version 1 E/X <BBpart> I20248</BBpart>
*GFP RBS Tester Version 2 S/X <BBpart> I20249</BBpart>
*RFP RBS Tester Version 1 E/X <BBpart> I20250</BBpart>
*RFP RBS Tester Version 2 S/X <BBpart> I20251</BBpart>
 
==Inverter/Terminator Tester==
[[Image:T9002.1.jpg|thumb|left|T9002 Design]]
*Based on <BBpart> T9002</BBpart>
 
*F2620________E X P1010 S P________E0040
*Contains a full BB site to allow construction within the device
<br style="clear:both" />


==References==
==References==
*[[Registry/Measurement kit/Notebook|Lab Notebook on construction of the kit]]
<biblio>
#Alper-PNAS-2005 pmid=16123130
#Zaslaver-PNAS-2006 pmid=16862137
#Chen-NAR-1994 pmid=7528374
</biblio>

Latest revision as of 15:53, 22 March 2008