Registry/Measurement kit
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The Measurement Kit consists of Promoter Testers, RBS Testers, and an Inverter/Terminator Tester. All Devices are on the 3K3 plasmid.
Promoter Tester
The Promoter Tester using GFP is based on <BBpart> E0240</BBpart> and using RFP is based on <BBpart> I2057</BBpart>. Both come in two versions.
- Version 1 (E/S)
- ___E X CCDB S___RBS GFP T
- Cut promoter E/S and insert
- Can take part back out if necessary
- Version 2
- ___E CCDB X___E0240
- Cut Promoter E/S to insert
- Forms mixed site so can't remove, real conditions
RBS Tester
The RBS Tester using GFP is based on <BBpart> I2055</BBpart> and using RFP is based on <BBpart> I2056</BBpart>. Both come in two versions.
- Versions 1 (E/X)
- __R0040___E CCDB X____GFP T
- To insert RBS cut E/S, forms a mixed Spe/Xba site
- Has too big spacing (full BB prefix) b/t promoter and RBS
- Easier for testers to use
- Version 2 (S/X)
- __R0040___S CCDB X____GFP T
- Will get 2 spe/xba mixed sites, can't get RBS out
- Cut RBS X/S, ligate w/ Spe1 and Xba1 restriction enzymes to ensure proper orientation
- Real condition spacing
Inverter/Terminator Tester
- Based on <BBpart> T9002</BBpart>
- F2620________E X P1010 S P________E0040
- Contains a full BB site to allow construction within the device