Registry/Measurement kit/Notebook: Difference between revisions
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#**Colony PCR and Seq more colonies | #**Colony PCR and Seq more colonies | ||
#Insert 2 promoters (strong/weak) and 2 RBS's (strong/weak) into each of the devices | #Insert 2 promoters (strong/weak) and 2 RBS's (strong/weak) into each of the devices | ||
#*B0034 and R0040 | #*weak: B0034 and R0040 | ||
#*B0031 and J23100 | #*strong: B0031 and J23100 | ||
==Summer Work== | ==Summer Work== |
Revision as of 06:49, 21 August 2007
Post Summer
- Finish construction/verification of the kit components
- <bbpart>I20244</bbpart>-GFP Promoter tester (E/S)
- Re-sequence E2-#3, which has the mutation repaired, but the sequencing didn't cover the BB site (no reason to expect it would have been changed by the site-directed mut. So it's probably correct.
- <bbpart>I20248</bbpart>-GFP RBS tester (E/X)
- Colony PCR and Seq more colonies (#2 and #3 should be PCR'd from glycerol stocks)
- <bbpart>I20249</bbpart>-GFP RBS tester (S/X)
- Colony PCR and Seq more colonies
- <bbpart>I20244</bbpart>-GFP Promoter tester (E/S)
- Insert 2 promoters (strong/weak) and 2 RBS's (strong/weak) into each of the devices
- weak: B0034 and R0040
- strong: B0031 and J23100
Summer Work
<calendar> name=Registry/Measurement_kit/Notebook date=2007/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>