Registry/Measurement kit/Notebook

Post Summer

1. Finish construction/verification of the kit components
   • <bbpart>I20248</bbpart>-GFP RBS tester (E/X)
     • Colony PCR and Seq more colonies (#2 and #3 should be PCR'd from glycerol stocks)
   • <bbpart>I20249</bbpart>-GFP RBS tester (S/X)
     • Colony PCR and Seq more colonies
2. Insert 2 promoters (strong/weak) and 2 RBS's (strong/weak) into each of the devices

Construction Progress

Promoter Testers

	J23116(weak)	R0040(strong)	Description
<bbpart>I20244</bbpart>	-	-	GFP (E/S)
<bbpart>I20245</bbpart>	-	-	GFP (E/X)
<bbpart>I20246</bbpart>	-		RFP (E/S)
<bbpart>I20247</bbpart>	-		RFP (E/X)

RBS Testers

	B0031(weak)	B0032(strong)	Description
<bbpart>I20248</bbpart>	-	-	GFP (E/X)
<bbpart>I20249</bbpart>		(have B0034)	GFP (S/X)
<bbpart>I20250</bbpart>	-	-	RFP (E/X)
<bbpart>I20251</bbpart>	-	-	RFP (S/X)

Completed

1. Finish construction/verification of the kit components
   • <bbpart>I20244</bbpart>-GFP Promoter tester (E/S)
     • Re-sequence E2-#3, which has the mutation repaired, but the sequencing didn't cover the BB site (no reason to expect it would have been changed by the site-directed mut. So it's probably correct.

Summer Work

<calendar> name=Registry/Measurement_kit/Notebook date=2007/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>