Registry/Measurement kit/Notebook: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
 
(38 intermediate revisions by 2 users not shown)
Line 1: Line 1:
==Post Summer==
==Post Summer==
#Finish construction/verification of the kit components
*[[Registry/Measurement kit/Preparation]]
#*<bbpart>I20244</bbpart>-GFP Promoter tester (E/S)
*[[Registry/Measurement kit/Instructions]]
#**Re-sequence E2-#3, which has the mutation repaired, but the sequencing didn't cover the BB site (no reason to expect it would have been changed by the site-directed mut.  So it's probably correct.
*[[Registry/Measurement kit/RBS Measurement Kit]]
#*<bbpart>I20248</bbpart>-GFP RBS tester (E/X)
*[[Registry/Measurement kit/Promoter Measurement Kit]]
#**
#*<bbpart>I20249</bbpart>-GFP RBS tester (S/X)
#**
#Insert 2 promoters (strong/weak) and 2 RBS's (strong/weak) into each of the devices
#*B0034 and R0040 (strong)
#*B0031 and J23100 (weak)


===Construction Progress===
{| border="1"
|+ Promoter Testers
! !! J23116(weak) !! R0040(strong) !! Description
|-
! <bbpart>I20244</bbpart>[[Image:Check.gif]]
| [[Image:Check.gif]] || [[Image:Check.gif]] || GFP (E/S)
|-
! <bbpart>I20245</bbpart>[[Image:Check.gif]]
| [[Image:Check.gif]] || [[Image:Check.gif]] || GFP (E/X)
|-
! <bbpart>I20246</bbpart>[[Image:Check.gif]]
| [[Image:Check.gif]] (reseq, has J23116)|| [[Image:Check.gif]] || RFP (E/S)
|-
! <bbpart>I20247</bbpart>[[Image:Check.gif]]
| [[Image:Check.gif]] || [[Image:Check.gif]] || RFP (E/X)
|-
|}
{| border="1"
|+ RBS Testers
! !! B0031(weak) !! B0032(strong) !! Description
|-
! <bbpart>I20248</bbpart>[[Image:Check.gif]]
| [[Image:Check.gif]] || [[Image:Check.gif]] || GFP (E/X)
|-
! <bbpart>I20249</bbpart>[[Image:Check.gif]]
| [[Image:Check.gif]]  || (have B0034) || GFP (S/X)
|-
! <bbpart>I20250</bbpart>[[Image:Check.gif]]
| [[Image:Check.gif]] || [[Image:Check.gif]] || RFP (E/X)
|-
! <bbpart>I20251</bbpart>[[Image:Check.gif]]
| - || [[Image:Check.gif]] || RFP (S/X)
|-
|}
*<bbpart>I20252</bbpart> - RFP positive control (not built)
*<bbpart>I7101</bbpart> - GFP positive contol [[Image:Check.gif]]


==Summer Work==
==Summer Work==
<calendar>
<calendar>
name=Registry/Measurement_kit/Notebook
name=Registry/Measurement_kit/Notebook
date=2007/07/01
date=2007/11/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>
 
==Continued 2008 ==
<calendar>
name=Registry/Measurement_kit/Notebook
date=2008/02/09
view=threemonths
view=threemonths
format=%name/%year-%month-%day
format=%name/%year-%month-%day
weekstart=7
weekstart=7
</calendar>
</calendar>
==Protocols==
*[[Registry/Measurement kit/Protocols/Plate reader experiment]]

Latest revision as of 06:09, 6 February 2008

Post Summer

Construction Progress

Promoter Testers
J23116(weak) R0040(strong) Description
<bbpart>I20244</bbpart> GFP (E/S)
<bbpart>I20245</bbpart> GFP (E/X)
<bbpart>I20246</bbpart> (reseq, has J23116) RFP (E/S)
<bbpart>I20247</bbpart> RFP (E/X)
RBS Testers
B0031(weak) B0032(strong) Description
<bbpart>I20248</bbpart> GFP (E/X)
<bbpart>I20249</bbpart> (have B0034) GFP (S/X)
<bbpart>I20250</bbpart> RFP (E/X)
<bbpart>I20251</bbpart> - RFP (S/X)
  • <bbpart>I20252</bbpart> - RFP positive control (not built)
  • <bbpart>I7101</bbpart> - GFP positive contol

Summer Work

<calendar> name=Registry/Measurement_kit/Notebook date=2007/11/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Continued 2008

<calendar> name=Registry/Measurement_kit/Notebook date=2008/02/09 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Protocols

Retrieved from "https://openwetware.org/mediawiki/index.php?title=Registry/Measurement_kit/Notebook&oldid=184499"