Registry/Measurement kit/Notebook/2007-6-17
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- As of noon today, there was no growth on the plates from the transfer.
Possible options:
- Plating the rest of the LB from the transfer
- Replica-plating, in case the desired colonies didn't get transferred at all
- Re-trying the transformation
- Going with option 1. Failing that, will try #3 tomorrow.
- Procedure for plating rest of the cells:
- Place in eppendorfs and spin at 4k for 4 minutes.
- Remove all but 100µL of the supernatant
- Plate.