Registry/Measurement kit/Notebook/2007-6-20: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 11: | Line 11: | ||
=="Device B" Primers== | =="Device B" Primers== | ||
Fwd | *Fwd | ||
MfeI tail + tccctatcagtgatagagattgacatc | **MfeI tail + tccctatcagtgatagagattgacatc | ||
Rev | *Rev | ||
NsiI tail + TATAAACGCAGAAAGGCCCAC | **NsiI tail + TATAAACGCAGAAAGGCCCAC | ||
Revision as of 11:45, 20 June 2007
To Do
- Add new devices into the Registry
- Check re-plating of devices A and C (done. no growth.)
- Create/order primers for device B
- Mini-prep and sequence device B culture (colony 8) and Jason's three cultures
Mini-Prep
- Spun at 5k for 4.5 min before prepping
- note: accidentally added 250 of P1 to each tube instead of each culture, but reconsolidated into four miniprep columns. We'll see if this affects the results drastically.
- Eluted with 30µL of EB.
"Device B" Primers
- Fwd
- MfeI tail + tccctatcagtgatagagattgacatc
- Rev
- NsiI tail + TATAAACGCAGAAAGGCCCAC
