Registry/Measurement kit/Notebook/2007-7-25: Difference between revisions
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==I2056== | ==I2056== | ||
*Ligating into 1AK3; ran out of 3K3. | *Ligating into 1AK3; ran out of 3K3. | ||
==I2055== | |||
*Re-trying promoter PCR | |||
==ccdB== | |||
*Started PCRs with E/X tails and S/X tails. | |||
==Transformations/overnight stuff== | ==Transformations/overnight stuff== | ||
Revision as of 11:52, 25 July 2007
T9002
- F2620 was ligated into 3K3(E/P) instead of E/X
- Re-ligated F2620 (M/X) into 3K3 (E/X) and 1AK3 (E/X)
- Reaction with 3K3 was left in incubator too long. Made another reaction, will transform both.
- Transformations went into warm room at 2:30
Misc
Also Digesting more 3K3 (E/P)Making o/n of 3K3- Mini-prepped 1AK3, digested E/X, E/P
I2056
- Ligating into 1AK3; ran out of 3K3.
I2055
- Re-trying promoter PCR
ccdB
- Started PCRs with E/X tails and S/X tails.
Transformations/overnight stuff
- O/n of 3K3
- Transformation of F2620-3K3 (2 samples), F2620-1AK3, and I2056-1AK3.
- Digesting 1AK3 E/X, E/P
