Registry/Measurement kit/Notebook/2007-7-4: Difference between revisions
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*Made glycerol stocks of colonies 3,4,6 and mini-prepping the remaining 4mL. | *Made glycerol stocks of colonies 3,4,6 and mini-prepping the remaining 4mL. | ||
**Will send for sequencing today. | **Will send for sequencing today. | ||
*Sequencing order: 3F, 3R, 4F, 4R, 6F, 6R | |||
==I2057== | ==I2057== | ||
*Sequence matches! | *Sequence matches! | ||
==T9002== | |||
*Sequential NsiI/MfeI digest. | |||
Revision as of 09:21, 4 July 2007
Colony PCR
- Yet again, yesterday's PCR seems to have evaporated...
- Trying again with a different tube.
E0240
- Made glycerol stocks of colonies 3,4,6 and mini-prepping the remaining 4mL.
- Will send for sequencing today.
- Sequencing order: 3F, 3R, 4F, 4R, 6F, 6R
I2057
- Sequence matches!
T9002
- Sequential NsiI/MfeI digest.
